相关实验视频

Updated: Sep 16, 2025

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

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使用DNA酶和定量RT-PCR在非编码RNA中的2'-O-甲基化残留物的定量检测

Valentyne Lebret-Kogey1, Lilia Ayadi2, Sylvain Maenner3

  • 1Université de Lorraine, CNRS, UMR7365 IMoPA, Nancy, France; ENS de Lyon, Lyon, France.

Biochimie
|July 9, 2025
PubMed
概括

这项研究引入了DAMP-RNA,一种基于DNA酶的方法,用于验证RNA中的2'-O-甲基化. 它提供了一种敏感和特定的方法来准确量化RNA修饰,改进表观转录学研究.

关键词:
通过2′-O-甲基化.这就是DNAzyme DNA酶.脱氧核糖酶是一种脱氧核糖酶.基因组RNA的修改 基因组RNA的改变我们的nRNARNA

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Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs08:49

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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