通过计算和实验方法揭示Curcuma amada根茎提取物对抗前列腺癌的抗癌潜力
Arpita Priyadarshini1, Debajani Mohanty1, Swagat Mohanty1
1Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinga Nagar, Bhubaneswar, 751003, India.
Scientific reports
|July 9, 2025
概括
库尔库马阿马达根茎提取物 (CARE) 显示出前列腺癌 (PCa) 治疗的前景. 网络药理学和体外研究表明,CARE通过PI3K-AKT通路抑制PCa细胞增殖并诱导细胞亡.
科学领域:
- 植物化学 植物化学
- 计算生物学 计算生物学
- 在瘤学瘤学.
背景情况:
- 前列腺癌 (PCa) 是男性的重大全球健康问题.
- 对于对抗PCa的Curcuma amada提取物的治疗机制尚不清楚.
- 自然化合物为新的癌症疗法提供了潜力.
研究的目的:
- 阐明Curcuma amada根茎提取物 (CARE) 在治疗PCa.的潜在机制.
- 整合网络药理学,生物信息学和体外验证.
- 确定PCa中CARE的关键活性化合物和分子标.
主要方法:
- 用于植物成分分析的UHPLC-QTOF-HRMS/MS.
- 网络药理学和生物信息学用于目标识别 (PPI,GO,KEGG).
- 分子对接,MD模拟和体外实验 (细胞增殖,细胞亡,qRT-PCR).
主要成果:
- 在CARE中确定了16种植物成分;CARE和PCa之间有59个重叠的目标.
- 确定了关键枢纽目标 (如P53,AKT1,EGFR) 和核心化合物.
- CARE抑制了PC-3细胞的增殖,诱导了细胞亡,并阻止了细胞循环.
- 在PI3K-AKT信号通路中,CARE抑制了mRNA表达.
结论:
- 通过调节关键分子标,CARE表现出抗前列腺癌的活性.
- PI3K-AKT信号通路是CARE抗PCa效应的关键目标.
- 在前列腺癌治疗中,CARE 是一种潜在的治疗剂.
关键词:
黄 (Curcuma amada) 是一种的植物.分子对接是分子对接.分子动力学模拟模型网络药理学 网络药理学前列腺癌是什么意思 前列腺癌是什么意思在UHPLC-QTOF-HRMS/MS中使用.更多相关视频
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