形束计算机断层扫描对不同年龄组的脱皮表皮细胞的基因毒性和细胞毒性影响
Maged Bakr1, Fatma Ata1, Asmaa Saleh Elmahdy2
1Department of Oral Medicine, Periodontology, Diagnosis and Oral Radiology, Faculty of Dentistry, Mansoura University, El- gomhouria Street, P.O. Box: 35516, Mansoura City, Dakahlia, Egypt.
BMC oral health
|July 9, 2025
概括
圆束计算机断层扫描 (CBCT) 在成人和儿童中都会导致DNA损伤和细胞损伤. 年轻的患者在CBCT辐射暴露后表现出更显著的细胞毒性作用.
科学领域:
- 牙科放射学 牙科放射学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 圆束计算断层扫描 (CBCT) 是一种广泛使用的牙科放射技术.
- 人们对CBCT辐射的生物效应存在担忧,特别是在儿科患者身上.
- 这项研究调查了CBCT对不同年龄组口腔上皮细胞的细胞毒性和基因毒性影响.
研究的目的:
- 评估CBCT辐射的细胞毒性和基因毒性影响.
- 为了比较这些效应在成人和儿科患者群体之间的脱皮口腔上皮细胞.
- 在CBCT程序后评估辐射诱导的细胞变化和DNA损伤.
主要方法:
- 40名患者被分为两个组:成年人 (≥18岁) 和儿童 (<18岁).
- 在CBCT暴露之前和10-12天后收集了皮的口腔上皮细胞.
- 用帕帕尼科劳法染色细胞学涂抹,以评估微核数量和细胞毒性变化 (化,皮克诺斯,性).
- 统计分析包括曼·惠特尼U测试和双向ANOVA.
主要成果:
- 在CBCT暴露后,在两个年龄组中观察到显著增加的微核数量和细胞毒性细胞变化.
- 在CBCT后的成人和儿科组之间没有发现这些参数的统计学上显著差异.
- 细胞毒性在年轻患者中更为明显,尽管在不同组之间没有统计学意义.
结论:
- 暴露于CBCT会在脱皮的口腔上皮细胞中诱导基因毒性和细胞毒性作用.
- 年轻患者在CBCT后表现出更高程度的细胞毒性.
- 这些发现强调了在使用CBCT之前需要仔细的临床证明的必要性,特别是在儿科牙科中.
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