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Updated: Sep 16, 2025

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
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The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Published on: May 25, 2018

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探索免疫蛋白酶的基质偏好,以改善水解和选择性

Christine S Muli1, Cody A Loy2, Darci J Trader1,2,3

  • 1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University 575 West Stadium Avenue West Lafayette Indiana 47907 USA.

RSC chemical biology
|July 10, 2025
PubMed
概括

研究人员确定了对结基质的修改,这些修改提高了免疫蛋白酶体 (iCP) 的选择性超过三倍. 这一发现有助于设计针对性的ICP探针,前药物和治疗应用的抑制剂.

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Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

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相关概念视频

The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
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The Proteasome Structure01:17

The Proteasome Structure

The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
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