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小麦内精特异性转录因子TaDOF6通过调节TaSWEET13h的表达和促进糖和吉伯雷林的运输来增强谷物发育

Run Ding¹, Tongtong Xiao¹, ShaSha Li¹

¹College of Life Science, Capital Normal University, Beijing, China.

Frontiers in plant science

|July 10, 2025

概括

在小麦谷物中过度表达TaDOF6会增加糖和吉伯雷林 (GA3) 水平,增加种子大小. 这突出了TaDOF6-TaSWEET13h路径作为提高小麦产量的目标.

科学领域:

植物生物学植物生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

种子大小对作物产量至关重要,并由复杂的遗传和分子机制调节.
植物激素,糖和细胞循环过程是种子发育的关键调节者.

研究的目的:

调查内精特异性DOF转录因子基因*TaDOF6*在调节小麦粒填充和大小中的作用.
确定TaDOF6的下游目标,并阐明底层的分子机制.

主要方法:

在小麦内精中过度表达基因.
用于基因表达分析的RNA测序 (RNA-seq) 和定量实时PCR (RT-qPCR).
酵母单杂交 (Y1H),EMSA和双露西法酶试验用于转录因子-向基因相互作用的验证.
结构和功能分析,包括分子动力学 (MD) 模拟,用于传送器特性.

主要成果:

在谷物填充过程中, *TaDOF6* 的过度表达显著增加了糖和吉伯雷林 (GA3) 积累.
*TaSWEET13h*被确定为TaDOF6.6的直接下游目标.
塔斯威特13h作为溶性糖和GA3的细胞膜载体,其水性相互作用驱动了糖和GA3的运输.

结论:

这项研究阐明了一种涉及TaDOF6和SWEET传递器*TaSWEET13h*控制小麦粒大小的遗传调节网络.
这条路径提出了通过育种策略提高小麦产量的有希望的目标.

关键词:

DOF转录因子的转录因子甜食运输蛋白质运输蛋白质分子动力学模拟的模拟.种子大小种子大小转录规则转录规则转录规则小麦小麦小麦小麦小麦小麦小麦.

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