压力测试的蛋白质组概况评估了心血管疾病风险易患糖尿病的受试者
Yugendra R Patil1,2, Shalbha Tiwari3, A G Unnikrishnan3
1Biochemical Sciences Division, CSIR-National Chemical Laboratory, Dr Homi Bhabha Road, Pashan, Pune, 411008, India.
研究人员确定了新型血蛋白 (CO4B,PON1,LUM) 作为糖尿病患者心血管疾病 (CVD) 风险的潜在生物标志物. 这些标记物可以作为评估心血管疾病风险的传统跑步机测试 (TMT) 的替代品.
科学领域:
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学
- 心血管医学 心血管医学
背景情况:
- 心血管疾病 (CVD) 是糖尿病患者的主要死亡原因.
- 准确预测糖尿病患者心血管疾病风险需要特定可靠的生物标志物.
- 像跑步机测试 (TMT) 这样的当前方法在评估心血管疾病风险方面存在局限性.
研究的目的:
- 确定用于评估糖尿病患者心血管疾病 (CVD) 风险的新型血蛋白生物标志物.
- 为了比较糖尿病压力测试阳性 (DSTP) 和糖尿病压力测试阴性 (DSTN) 患者的血蛋白质组概况.
- 通过目标质谱测量验证潜在的CVD生物标志物.
主要方法:
- 利用基于SWATH-MS的无标签定量质谱法来分析等离子体蛋白质组.
- 在DSTP和DSTN组之间确定了差异表达蛋白 (DEP).
- 使用向质谱 (MRM-HR) 和ROC分析验证了关键的CVD相关的DEP.
主要成果:
- 鉴定了CO4B,PON1和LUM作为糖尿病患者具有不同心血管疾病风险的显著差异表达的蛋白质.
- 通过ROC分析实现了0.97的CO4B,0.79的PON1和0.77的LUM曲线下的显著面积 (AUC) 值.
- 在两种定量质谱方法中证明了这些蛋白质的一致差异表达.
结论:
- 建议CO4B,PON1和LUM作为评估糖尿病人群中心血管疾病风险的潜在替代生物标志物.
- 这些已识别的蛋白质可以帮助克服与传统跑步机测试 (TMT) 相关的局限性.
- 建议在更大的队列中进一步验证,以确认这些新生物标志物的临床实用性.
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