Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.3K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.3K
Labeling DNA Probes03:31

Labeling DNA Probes

8.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Inflammatory cell death and monocyte dysfunction in VEXAS syndrome.

Blood·2026
Same author

Decoding the host-pathogen proteomic dialogue using surface labeling tools.

FEBS letters·2026
Same author

Proteomic analysis of the host-pathogen interface in experimental cholera.

Nature chemical biology·2021
Same author

Type I interferon remodels lysosome function and modifies intestinal epithelial defense.

Proceedings of the National Academy of Sciences of the United States of America·2020
Same author

Role and Recruitment of the TagL Peptidoglycan-Binding Protein during Type VI Secretion System Biogenesis.

Journal of bacteriology·2019
Same author

Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex.

Nature microbiology·2017
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: Sep 16, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.6K

使用近距离标记在感染期间探索细菌表面蛋白质组动力学.

Oceane Dubois1, Abdelrahim Zoued2

  • 1CIRI, Centre International de Recherche en Infectiologie, CNRS UMR5308, Inserm U1111, Université Claude Bernard Lyon 1, ENS de Lyon, Lyon, France.

Methods in molecular biology (Clifton, N.J.)
|July 10, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了依赖近距离的蛋白质生物化,以在感染期间映射细菌表面蛋白和宿主结合蛋白. 这种方法增强了对宿主-病原体相互作用的理解,并确定了潜在的治疗点.

关键词:
在APEX2中,APEX2是APEX2的第二个版本.汽车运输工具 汽车运输工具细菌表面标签的使用方法主体衍生的细菌结合蛋白 (HBBP)宿主细菌的相互作用第五类分泌系统 (T5SS)

更多相关视频

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

6.9K
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
07:40

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Published on: October 29, 2016

11.2K

相关实验视频

Last Updated: Sep 16, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.6K
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

6.9K
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
07:40

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Published on: October 29, 2016

11.2K

科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 传染性疾病 传染性疾病

背景情况:

  • 在感染期间研究细菌表面蛋白质对于理解宿主-病原体相互作用至关重要.
  • 现有的方法可能在准确地描述细菌表面蛋白质体的局部特征方面存在局限性.

研究的目的:

  • 开发和验证一种依赖近距离的蛋白质生物化方法,用于研究细菌表面蛋白质组.
  • 在感染期间识别细菌表面蛋白和宿主衍生细菌结合蛋白 (HBBPs).

主要方法:

  • 工程细菌自传递器以显示APEX2酶在细菌表面.
  • 实施一个工作流程,包括细菌培养,靠近依赖的生物化和免疫光标签.
  • 使用蛋白质分析技术来识别标记的蛋白质.

主要成果:

  • 成功标记和表征细菌表面蛋白质.
  • 鉴定与病原体相互作用的宿主衍生细菌结合蛋白 (HBBPs).
  • 对宿主-病原体相互作用研究的全面工作流程的演示.

结论:

  • 表面暴露的近距离标签为剖析细菌病变产生提供了一个强大的工具.
  • 这种方法可以揭示新的毒性因素和治疗点.
  • 该方法可以适应各种细菌物种和感染模型.