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Updated: Sep 16, 2025

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在Arabidopsis中用于基于TurboID的近距离标记质谱的生物化蛋白质的检测和量化

Sumudu S Karunadasa1,2, TaraBryn S Grismer1,2, Wenxuan Zhai1,2

  • 1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA.

Methods in molecular biology (Clifton, N.J.)
|July 10, 2025
PubMed
概括

与质谱学 (PL-MS) 相结合的TurboID近距离标签识别可以识别相互作用的蛋白质. 本协议详细介绍了对Arabidopsis的样本制备和用于蛋白质识别的数据分析.

关键词:
这是LC-MS/MS.没有标签的量化 (LFQ)蛋白相互作用网络是蛋白质的相互作用网络.近距离标记质谱法 (PL-MS) 的使用在Arabidopsis (SILIA) 中稳定同位素标记.这是一种Streptavidin缩剂.这是一个TurboIDID.

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