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Updated: Sep 16, 2025

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
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在Arabidopsis中用于基于TurboID的近距离标记质谱的生物化部位的检测和量化

Sumudu S Karunadasa1,2, TaraBryn S Grismer1,2, Andres V Reyes1,2

  • 1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA.

Methods in molecular biology (Clifton, N.J.)
|July 10, 2025
PubMed
概括

这项研究详细介绍了用于近距离标记质谱法 (PL-MS) 的类水平丰富协议. 它改善了生物化的检测,这对于绘制蛋白质相互作用和蛋白质组的映射至关重要.

关键词:
生物化丰富剂 生物化丰富剂没有标签的量化 (LFQ)近距离标记质谱法 (PL-MS) 的使用在Arabidopsis (SILIA) 中稳定同位素标记.这是一种Streptavidin缩剂.这是一个TurboIDID.

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