相关实验视频

Updated: May 3, 2026

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line

Published on: May 10, 2017

转录组分析揭示了参与Peucedanum praeruptorum Dunn 多糖诱导的淋巴细胞激活的关键基因表达和信号通路

Ming Zhao¹, Shiyue Qin¹, Yushi Wang¹

¹Department of Veterinary Medicine, MOA Key Laboratory of Animal Virology, Center for Veterinary Sciences, College of Animal Sciences, Zhejiang University, Hangzhou, China.

Chemistry & biodiversity

|July 10, 2025

概括

(Peucedanum praeruptorum Dunn,PPD) 的多糖可显著增强淋巴细胞活动和免疫反应. 这些化合物调节T细胞子集,并激活MAPK和JAK-STAT等关键信号通路.

科学领域:

免疫学免疫学免疫学
药理学药理学是一个学科.
分子生物学分子生物学

背景情况:

多糖是Peucedanum praeruptorum Dunn (PPD) 中的主要活性成分.
PPD多糖的免疫调节功能和分子机制在很大程度上仍未被定义.

研究的目的:

研究PPD多糖在淋巴细胞上的免疫调节作用.
阐明PPD免疫调节作用的潜在分子机制.

主要方法:

淋巴细胞增殖试验和细胞因子分泌分析 (IFN-γ,IL-12,IL-6,IL-10,TNF-α,IgG).
流细胞计分析T细胞子集比例.
西部涂抹以评估MAPK和JAK-STAT通路的酸化.
RNA测序 (RNA-seq) 用于识别差异表达的基因和丰富的通路 (GO,KEGG).
途径抑制剂实验以验证信号途径的参与.

主要成果:

PPD多糖在很大程度上促进了淋巴细胞的增殖和关键细胞因子 (IFN-γ,IL-12p40,IL-6,IL-10,TNF-α) 和IgG的分泌.
PPDs调节了T细胞子集的比例,并增强了p38,JNK,ERK,STAT1和STAT3.3的酸化.
RNA-seq揭示了5951个差异表达的基因,2561个基因本体学术语和81个KEGG通路.
特定途径的抑制剂抑制了PPD刺激的TNF-α和IL-6的产生.

结论:

PPD多糖对淋巴细胞具有显著的免疫调节特性.
观察到的效应与MAPK和JAK-STAT信号通路的激活密切相关.

关键词:

皮塞丹姆 (Peucedanum praeruptorum) 是丹恩的多糖类之一.免疫调节免疫调节免疫调节淋巴细胞的淋巴细胞.信号通道的信号通道.

更多相关视频

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

相关概念视频

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...