相关实验视频

Updated: Sep 16, 2025

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
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Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Published on: July 20, 2022

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在RNA病毒复制中照亮可用药物的暗物质,使用in situ冷EM

Himanshu Sharma1, Marie N Sorin2, Kiran B Sharma2

  • 1Department of Medical Biochemistry and Biophysics, Umeå University, Sweden; Wallenberg Centre for Molecular Medicine, Umeå University, Sweden; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, Sweden; SciLifeLab, Umeå University, Sweden; Department of Chemistry, Faculty of Science and Technology, NOVA University, Lisbon.

Current opinion in virology
|July 10, 2025
PubMed
概括

在位冷电子显微镜 (cryo-EM) 可视化宿主细胞内的病毒蛋白,揭示RNA病毒复制的关键结构. 这一技术有助于开发新的抗病毒药物和策略.

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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