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Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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相关实验视频

Updated: Sep 16, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

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为了新的育种技术,进行高效和多用途的菜种子转化.

Kea Ille1, Siegbert Melzer1

  • 1Plant Developmental Biology and Physiology, Kiel University, Am Botanischen Garten 5, 24118, Kiel, Germany.

The Plant journal : for cell and molecular biology
|July 10, 2025
PubMed
概括

我们开发了一种新的方法,可以有效地转化冬季大麻,克服再生挑战. 这可以通过同时编辑多个同源基因,在这个重要的作物中更快地进行基因功能研究.

科学领域:

  • 植物生物技术 植物生物技术
  • 农作物科学 农作物科学
  • 分子生物学分子生物学

背景情况:

  • 阿拉比多普西斯基因功能知识广泛,但由于转化限制,很难将其应用于作物.
  • 冬季大菜 (Brassica napus L.) 对体外再生和转化具有反抗性,阻碍了基因功能分析.
  • 布拉西卡纳普斯 (Brassica napus) 的全四形性质和该科的基因组三倍化使得基因功能研究复杂,因为它有许多同类物种.

研究的目的:

  • 为了建立一个高效的转化和再生方法,冬季大麻.
  • 为了使在Brassica napus.中同时进行基因家族编辑和功能分析.
  • 克服研究反抗性作物物种基因功能的局限性.

主要方法:

  • 利用来自Beta vulgaris的WUSCHEL基因来增强冬季大麻的再生.
  • 采用Agrobacterium介导的转换,用于冬季和春季的菜种子基因型.
  • 应用CRISPR/Cas9基因编辑以准BnCLV3和BnSPL9/15基因家族.

主要成果:

  • 成功建立了一个高效的转化协议,用于冬季大菜的再生.
  • 在Brassica napus.中证明了整个基因家族 (BnCLV3,BnSPL9/15) 的同时编辑.
关键词:
布拉斯卡纳普斯 (Brassica napus) 是一种植物.在 CLV3 中使用.克里斯普尔是什么意思?克里斯普尔是什么意思?在SPL15中使用SPL15.在SPL9中使用.这就是WUSCHEL.强硬的冬季焦油菜种子转化转化转化转化转化转化转化转化

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  • 在初级变形体中观察到突变的表型,表明多达八个同源基因的双基因淘汰成功.

    • 结论:

    • 开发的方法显著提高了在冬季大麻的基因功能研究的效率.
    • 该协议有助于快速鉴定Brassica napus.中的基因家族,包括多余的同类基因.
    • 沃舍尔辅助的转换系统为改善作物和布拉西卡系植物的功能基因组学提供了强大的工具.