评估大气冷等离子体和微波辐射对聚甲氨酸酶的活动,稳定性,动力学和结构变化的影响
Jayashree B Potkule1, Suraj P Kahar1, Uday S Annapure1
1Department of Food Engineering and Technology, Institute of Chemical Technology, Matunga, Mumbai, India.
International journal of biological macromolecules
|July 10, 2025
概括
大气中的冷等离子体和微波辐射显著增强了多甲氨酶的活性和稳定性. 这些治疗诱导结构变化,增加β片和蛋白质聚合,以改善酶特性.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 蛋白质化学 蛋白质化学
背景情况:
- 聚甲氨酸酶对于通过化甘氨酸结合物来使pectin脱聚变至关重要.
- 了解酶修饰是优化工业应用的关键.
研究的目的:
- 为了比较评估大气冷等离子体和微波辐射对多甲酶的影响.
- 为了研究酶活性,稳定性,动力学和结构的变化.
主要方法:
- 使用大气冷等离子体和微波辐射进行酶处理.
- 分析酶活性,硫基,紫外线可见吸收,光和水性.
- 光谱分析 (CD,FTIR) 用于确定二级和三级结构变化.
主要成果:
- 观察到相对多聚氨酸酶活性显著增加 (129%和193%).
- 结构性修改包括减少的自由硫基,增加的二硫化物连接,增强的聚合,以及改变的二次结构 (增加β-sheet,减少α-helix).
- 紫外线可见的吸收率,光度和水性变化表明了三级结构变化.
结论:
- 大气冷等离子体和微波辐射都有效地改善了多甲氨酶的特性.
- 修改的程度取决于处理参数和酶特性.
- 诱导的结构变化,特别是增加的β片和聚合,有助于提高酶的效率和稳定性.
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