Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Programmable mRNA 3'UTR engineering restores MHC-I and overcomes immune evasion in prostate cancer.

Nature biomedical engineering·2026
Same author

CRISPR-engineered human GATA2 deficiency model uncovers mitotic dysfunction and premature aging in HSPCs, impairing hematopoietic fitness.

Leukemia·2025
Same author

BMAL1-HIF2A heterodimer modulates circadian variations of myocardial injury.

Nature·2025
Same author

Enhancer RNA Transcriptome-Wide Association Study Reveals a Distinctive Class of Pan-Cancer Susceptibility eRNAs.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Impact of rare non-coding variants on human diseases through alternative polyadenylation outliers.

Nature communications·2025
Same author

Modular organization of enhancer network provides transcriptional robustness in mammalian development.

Nucleic acids research·2025
Same journal

Sub1 contributes to heart failure with preserved ejection fraction driven by aging in mice.

Nature communications·2026
Same journal

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

Nature communications·2026
Same journal

Signaling downstream of tumor-stroma interaction regulates mucinous colorectal adenocarcinoma apicobasal polarity.

Nature communications·2026
Same journal

Click-polymerized polyenamine membranes for efficient lithium extraction.

Nature communications·2026
Same journal

Joint trajectories of brain atrophy, white matter hyperintensities and cognition quantify brain maintenance.

Nature communications·2026
Same journal

Proton shuttling at electrochemical interfaces under alkaline hydrogen evolution.

Nature communications·2026
查看所有相关文章

相关实验视频

Updated: Sep 16, 2025

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.1K

使用SCALPEL在单细胞水平上对转录异型的量化.

Franz Ake1,2, Marcel Schilling1,3, Sandra M Fernández-Moya1,4

  • 1Gene Regulation of Cell Identity Lab, Neurosciences Program, Bellvitge Institute for Biomedical Research (IDIBELL), L'Hospitalet del Llobregat, Spain.

Nature communications
|July 10, 2025
PubMed
概括
此摘要是机器生成的。

SCALPEL通过从3'数据中准确量化转录异型来增强单细胞RNA测序 (scRNA-seq). 该工具揭示了新的细胞群和调控机制,改进了转录组分析.

更多相关视频

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.7K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K

相关实验视频

Last Updated: Sep 16, 2025

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.1K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.7K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 单细胞RNA测序 (scRNA-seq) 对于研究细胞转录组多样性至关重要.
  • 现有的scRNA-seq方法通常在异形量化中的灵敏度和准确性方面扎.
  • 鉴定转录异型的特征对于理解转录后基因调节至关重要.

研究的目的:

  • 介绍SCALPEL,这是一个基于Nextflow的工具,用于从3'scRNA-seq数据中量化和表征转录异型.
  • 用合成和现实世界的数据集来评估SCALPEL的性能与现有方法相比.
  • 为了证明SCALPEL在发现新型细胞群和监管见解方面的实用性.

主要方法:

  • 开发SCALPEL,用于异形分析的Nextflow管道.
  • 使用合成scRNA-seq数据来对Scalpel的敏感性和特异性进行基准测试.
  • 将SCALPEL应用于用于生物发现的真实scRNA-seq数据集.
  • 将SCALPEL与配对的长短读取scRNA-seq数据集成在一起.

主要成果:

  • 与合成数据上的其他工具相比,SCALPEL具有更高的灵敏度和特异性.
  • SCALPEL的预测与现有方法和实验验证实数据集的实验验证有很高的一致性.
  • 在SCALPEL中,我们发现了新型细胞群体,这些细胞群体在标准基因表达分析中被遗漏了.
  • 在分化过程中,SCALPEL证实了3' UTR长度的变化,并发现了细胞类型特定的miRNA调控特征.
  • 在使用配对的长读和短读scRNA-seq数据时,SCALPEL提高了异形量化.

结论:

  • SCALPEL是一个灵敏而准确的工具,用于从3'scRNA-seq数据中量化异型.
  • SCALPEL扩展了scRNA-seq的功能,用于探索转录后基因调节.
  • 该工具有助于更深入地了解在不同生物环境中单细胞水平的基因调节机制.