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Updated: Sep 16, 2025

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

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在RanBP2,SUMO1-RanGAP1,E2 Ubc9,Crm1和Ran GTPase之间的核素出口复合物的结构基础

Vladimir Baytshtok1, Michael A DiMattia1,2, Christopher D Lima3,4

  • 1Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY, USA.

Nature communications
|July 10, 2025
PubMed
概括

这项研究揭示了核孔蛋白RanBP2如何与SUMO1修饰的RanGAP1和Ubc9.9相互作用. 这些相互作用对核运输和线粒细胞的进展至关重要,正如冷-EM结构所示.

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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