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使用MOF聚多巴胺纳米颗粒用于生物杀虫剂应用的增强和协同RNAi传递

Zhou Gao¹, Christopher Rensing², Jie Wang³

¹School of Agriculture and Biotechnology, Sun Yat-sen University, Shenzhen, PR China.

Nature communications

|July 10, 2025

概括

使用纳米颗粒的RNA干扰 (RNAi) 通过改善dsRNA吸收和调节肠道细菌来增强Spodoptera frugiperda中的害虫控制. 这种环保的方法可以提高植物的防御能力,并抑制病原体的生长.

科学领域:

农业科学农业科学
纳米技术纳米技术
昆虫学昆虫学是一门学科.

背景情况:

RNA干扰 (RNAi) 为化学农药提供了一个环保的替代方案.
抗击像Spodoptera frugiperda (S. frugiperda) 这样的类虫害的RNAi的有效性受到双链RNA (dsRNA) 吸收不佳的阻碍.
开发有效的传递系统对于推进基于RNAi的害虫管理策略至关重要.

研究的目的:

开发基于纳米粒子的传递系统,以增强S. frugiperda的dsRNA吸收.
研究纳米启用dsRNA对昆虫肠道微生物群和免疫反应的影响.
评估这种方法在综合性害虫防治方面的协同作用潜力.

主要方法:

合成ZIF-8聚多巴胺纳米颗粒以封装和保护dSRNA.
评估了纳米粒子介导的dsRNA传递及其对S. frugiperda的内细胞/体通路的影响.
分析了肠道细菌群 (Serratia marcescens,Enterococcus mundtii) 和反应性氧物种 (ROS) 治疗后水平的变化.

主要成果:

ZIF-8聚多巴胺纳米颗粒成功地保护dsrna免受降解,并通过内细胞路径增强其吸收.
纳米启用dsrna的摄入导致了昆虫肠道中serratia marcescens的过度生长.
这种微生物转移抑制了S.frugiperda ROS免疫反应,并抑制了Enterococcus mundtii的生长.

结论:

纳米粒子介导的dsRNA传递是一种有前途的战略,可以克服RNAi害虫控制中的吸收限制.
这种方法通过操纵昆虫肠道微生物群并减少免疫抑制来协同增强植物防御能力.
这项研究突出了纳米颗粒在综合性害虫防治方面的潜力,通过防止农药耐药性和改善生物控制来实现这一目标.

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