相关实验视频

Updated: Sep 16, 2025

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Published on: March 23, 2021

在肝细胞癌中,Clonorchis sinensis感染重塑了染色质的可访问性

Weilong Yang¹, Caibiao Wei², Junxian Chen²

¹Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

Parasites & vectors

|July 10, 2025

概括

在肝细胞癌 (HCC) 中,Clonorchis sinensis感染重塑了染色质的可访问性,促进了瘤的进展和细胞迁移. 这项研究揭示了与寄生性肝癌相关的表观遗传变化,有助于未来的治疗点发现.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
癌症生物学癌症生物学
寄生虫学的寄生虫学

背景情况:

肝细胞癌 (HCC) 是一个重要的全球健康问题.
克洛诺基斯·辛内斯 (Clonorchis sinensis) 感染是一种已知的致癌物,与肝病和HCC进展有关.
驱动C. sinensis相关的HCC的特定表观遗传机制尚未完全理解.

研究的目的:

调查染色质可访问性在C. sinensis相关的HCC中的作用.
确定表观遗传变化及其与C. sinensis感染个体HCC进展的关联.
评估C. sinensis分泌/分泌产品 (CsESPs) 对HCC细胞迁移的影响.

主要方法:

在C. sinensis感染和未感染的HCC瘤上进行ATAC-seq和RNA-seq.
集成ATAC-seq,RNA-seq和ChIP-seq数据来分析染色质可访问性,TF动图和基因组修饰.
利用伤试验来评估CsESPs对HCC细胞迁移的影响.

主要成果:

在C. sinensis+HCC瘤中确定了9,396个差异可访问区域 (DAR).
在DAR中发现了关键转录因子 (TFs) 的丰富,如HNF4A,FOXO1,ELF4和RELA.
观察到在新陈代谢,免疫调节和细胞骨动态中的差异性基因表达,与染色质可访问性和基因组修饰有关.
由于C. sinensis感染,证实了HCC细胞迁移能力的增加.

结论:

菌感染改变了染色质的可访问性,导致HCC的进展.
提供了有关寄生虫感染相关的HCC.CC.病变发生的见解.
建立了一个发现新生物标志物和C. sinensis相关HCC的治疗点的基础.

关键词:

这种动物叫Clonorchis sinensis.染色体的可访问性染色体的可访问性肝细胞癌是肝细胞癌.转录因子是一种转录因子.瘤的进展情况.

更多相关视频

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Published on: April 1, 2021

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

相关概念视频

Inheritance of Chromatin Structures

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...