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通过删除iolR和代谢工程在缺乏PTS的Corynebacterium glutamicum菌株中对石基酸盐产生的影响

Rui Wen Ou^1,2, Charles A Swofford^1,2, En-Ze Linda Zhong-Johnson¹

¹Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, United States.

Frontiers in bioengineering and biotechnology

|July 11, 2025

概括

在缺乏转移酶系统 (PTS) 的Corynebacterium glutamicum菌株中删除myo-inositol利用转录调节器 (iolR) 显著改善了细胞生长和shikimate生产. 这种代谢工程方法可以提高shikimate产量以获得有价值的衍生物.

科学领域:

微生物学微生物学
代谢工程是代谢工程.
生物技术是生物技术.

背景情况:

石基酸是合成高价值化学衍生物的关键前体.
细菌生产的shikimate经常使用非光转移酶系统 (非PTS).
在转移酶系统 (PTS) 缺陷菌株中,myo-inositol利用转录调节器 (iolR) 删除对shikimate产生的影响仍然未被探索.

研究的目的:

在一个缺乏PTS的菌株中,对Corynebacterium glutamicum进行工程改造,以提高shikimate的生产.
为了研究iolR缺失对PTS缺乏的C. glutamicum细胞生长和shikimate标位的影响.
通过代谢工程策略优化石基酸盐的生产.

主要方法:

消除光转移酶系统 (PTS) 以增加聚酸水平.
删除myo-inositol利用转录调节器 (iolR) 以改善生长和生产.
过度表达的基因在糖解和石基酸盐的途径.

主要成果:

消除PTS最初减少了细胞生长和石基酸盐的产生.
随后在缺乏PTS的菌株中进行了iolR删除,恢复并增强了细胞生长和shikimate生产.
综合的代谢工程策略产生了0.76 mg/mg DCW的石基酸盐含量和4.1 g/L的标位.

结论:

删除iolR对于改善PTS缺乏C. glutamicum的细胞生长和神基酸盐生产至关重要.
甲基工程的C. glutamicum,包括iolR删除和通路基因过度表达,有效地提高了shikimate产量.
这项研究为进一步的工程工作提供了宝贵的见解,以促进石基及其衍生物的生产.

关键词:

在Corynebacterium glutamicum的研究中,我们发现在PTS中存在缺陷.这就是iolRR的意思.代谢工程是代谢工程.什基马特 (shikimate) 是一种神经质的物质.

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