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开发用于玉米精密育种的氨酸和氨酸基编辑器

Xiao Fu¹, Nan Wang¹, Lina Li^1,2

¹State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Journal of integrative plant biology

|July 11, 2025

概括

优化基础编辑器显著提高了玉米基因组编辑效率. 这一突破提高了除草剂耐受性和作物的精确育种.

科学领域:

农业生物技术农业生物技术
植物遗传学植物遗传学
分子生物学分子生物学

背景情况:

基础编辑 (BE) 提供了提高作物的潜力,但其在玉米中的应用面临效率挑战.
开发精确高效的基因组编辑工具对于推进玉米基因工程至关重要.

研究的目的:

评估优化的细胞因子和腺因基编辑器 (CBEs和ABEs) 以提高玉米的基因组编辑.
为了提高编辑效率和可传承性,使用种子光报告器 (SFR) 系统.
评估基准编辑对玉米除草剂耐药性的影响.

主要方法:

在短暂和稳定的玉米细胞表达系统中测试了优化基编辑器,包括evoAPOBEC1,evoFERNY,evoCDA1,Tada8.20和Tada8e.
使用种子光报道器 (SFR) 系统快速选基编辑器变换剂和无转基因后代.
进行了ZmACC1/2基因的精确编辑,以评估对除草剂耐药性的影响.

主要成果:

实现了高的多重编辑效率,TadA8.20在测试位置达到100.0%,evoCDA1在测试位置达到79.0%.
产生的同卵性和双基突变,编辑效率分别超过72.4%和73.7%.
通过精确编辑ZmACC1/2,特别是ZmACC2突变,在玉米中证明了提高除草剂耐药性.

结论:

优化基础编辑器显著提高了玉米基因组编辑精度和效率.
种子光记者系统有效地加快了查,并识别了无转基因后代.
在不损害农学性能的情况下成功进行多地点修改,为改善玉米的除草剂耐受性和精确育种铺平了道路.

关键词:

齐亚梅斯 (Zea mays) 是一种植物.在 ZmACCC 里面,你会看到 ZmACC.氨酸 BE BE 的使用.细胞因子 BE BE 细胞因子抗除草剂耐药性抗除草剂耐药性多重编辑多重编辑编辑

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