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利多卡因在培养的人类肌细胞中影响原蛋白分解而不会损害细胞活力:一个体外研究

Filippo Randelli¹, Manuel G Mazzoleni¹, Alessandra Menon^2,3

¹Hip Department (CAD), Gaetano Pini-CTO Orthopedic Institute, University of Milan, P-za Cardinal Ferrari 1, 20122 Milano, Italy.

|July 11, 2025

概括

像利多卡因这样的局部麻醉剂不会损害细胞活力或原蛋白合成. 然而,利多卡因可能会增加原体的降解,而甲酸并不能防止这种氧化应激.

科学领域:

生物材料科学生物材料科学
细胞生物学细胞生物学
整形外科手术整形外科手术

背景情况:

局部麻醉剂 (LAs) 在周注射中使用,但人们对其毒性存在担忧.
利多卡因 (LD) 是一种常见的LA,在体外显示出细胞毒性作用.
了解LD对细胞和潜在保护剂的影响对于安全的临床使用至关重要.

研究的目的:

为了研究利多卡因对人类肌细胞亡,氧化应激和原循环的作用.
评估亚酸 (AA) 对LD诱导的细胞损伤的保护潜力.

主要方法:

人类肌细胞被用不同度的利多卡因 (0.2和1毫克/毫升) 和酸 (50和250微克/毫升) 治疗.
通过核形态,细胞染色体c和caspase 3激活来评估亡.
使用分子技术分析了原周转率,氧化应激 (HO-1) 和ECM稳态.

主要成果:

利多卡因没有诱导亡,也没有改变细胞中的原蛋白合成和成熟.
在利多卡因治疗后观察到原蛋白降解的增加.
亚酸并没有保护肌细胞免受LD诱导的氧化应激.

结论:

周内注射的利多卡因看起来是安全的,因为它对细胞的活力和原蛋白的合成是安全的.
利多卡因可能会促进原体的降解,而 Askorbic 酸的保护作用仍然不确定.
这项研究为利多卡因在肌治疗中的安全临床应用提供了有价值的数据.

关键词:

灭症 (apoptosis) 是一种死亡的过程.原体的营业额透的潜入方式利多卡因注射利多卡因注射肌病变是一种肌病变.肌肌是一种肌.十细胞细胞 (tenocytes) 是一种细胞.

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