在Corynebacterium rhinophilum sp. 的研究中, nov.从人类上呼吸道中分离出来
Elena B Popowitch1, Sefelani C Boiditswe2, Mohamed Z Patel3
1Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA.
概括
从人类呼吸道样本中确定了一种新的Corynebacterium物种Corynebacterium rhinophilum. 这一发现扩大了我们对Corynebacterium属及其在呼吸系统健康方面的潜在作用的理解.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 基因组学就是基因组学.
背景情况:
- 来自美国和博茨瓦纳的人类呼吸道样本产生了12种新的Corynebacterium菌株.
- 这些菌株代表Corynebacteriaceae家族中的潜在新物种.
研究的目的:
- 从人类呼吸道中分离出来的新型Corynebacterium菌株的特征和分类.
- 为了确定这些分离物的遗传学和基因组特征.
主要方法:
- 对五种菌株进行了表型和化学分类学分析.
- 对所有12个菌株进行了家族遗传学分析,包括16S核糖体RNA基因测序和全基因组测序.
- 计算了平均核酸标识 (ANI) 和in silico DNA-DNA杂交 (dDDH).
主要成果:
- 这些菌株是格拉姆阳性,非运动性细菌,在补充油酸的介质中具有最佳生长.
- 遗传学分析将这些菌株置于Corynebacterium属内,与C. accolens和C. macginleyi.yi.密切相关.
- 基因组数据 (2.47-2.62 Mbp,58.7-59.0% G+C含量) 和ANI/dDDH值 (93.19-93.62% ANI,19.9-36.3% dDDH) 证实了它们作为新物种的地位.
结论:
- 描述的菌株代表了一种新物种,被提出为Corynebacterium rhinophilum sp. 在新的一年里.
- 类型菌株是MSK072T (=DSM 118652T=JCM 37534T) 的.
- 这一发现有助于Corynebacterium属的分类学多样性.
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