推进真菌遗传学:整合现代测序,暗种群发现和机器学习
Syed Atif Hasan Naqvi1, Aqleem Abbas2, Ammarah Hasnain3
1Department of Plant Pathology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, 60800, Punjab, Pakistan. atifnaqvi@bzu.edu.pk.
Archives of microbiology
|July 11, 2025
概括
像DNA指纹,下一代测序 (NGS) 和第三代测序 (TGS) 等先进的分子技术正在彻底改变真菌诊断和疾病管理. 这些方法改善了物种识别和对真菌进化和病原性的理解.
科学领域:
- 菌类学 菌类学是指菌类学.
- 遗传学 遗传学是一种遗传学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 真菌遗传学取得了重大进展,影响了我们对真菌多样性,进化和病变的理解.
- 传统的基于形态学的分类学在解决神秘的物种化和非繁殖的真菌阶段方面面临着局限性.
研究的目的:
- 合成尖端的分子技术用于真菌诊断.
- 评估分子标记物和测序技术,用于物种识别,遗传学重建和疾病管理.
- 概述未来的真菌诊断和疾病缓解的方向.
主要方法:
- 分子技术的审查和合成,包括DNA指纹,下一代测序 (NGS) 和第三代测序 (TGS).
- 评估分子标记物,如内部转录间隔器 (ITS),大子单元 (LSU) 和蛋白质编码基因 (RPB1,RPB2,TEF1-α).
- 整合多omics方法和机器学习算法.
主要成果:
- ITS是真菌条形码的黄金标准;多位置策略提高了具有挑战性的一般的解决方案.
- NGS和TGS克服了传统的局限性,使高通量基因组分析和复杂的基因组组装成为可能.
- 进步促进了对真菌适应,病原性和宿主-病原体相互作用的洞察.
结论:
- 分子技术和多组学方法对于推进真菌诊断和分类学清晰度至关重要.
- 跨学科的合作和数据共享对于将基因组见解转化为精密疗法至关重要.
- 需要创新的工具和策略来减轻真菌病的影响,特别是在气候变化下.
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