在Streptomyces sp.中的生物合成. NRRL S-1813 和氧沙胺 A 和 A2 之间的调节
Qingyun Zhang1, Daotong Lv1, Huiyu Gong1
1College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
概括
研究人员优化了Streptomyces sp. 这种菌种. NRRL S-1813发酵以产生抗微生物二次代谢产物. 他们发现了来自氧化胺A的非酶形式的氧化胺A2,指导了有针对性的生产策略.
科学领域:
- 微生物学 微生物学
- 自然产品化学 自然产品化学
- 生物化学 生物化学
背景情况:
- 像Streptomyces这样的动态菌株是多样化二次代谢物具有抗菌性质的多产生产者.
- 密码基因集群的激活对于发现新生物活性化合物至关重要.
- 优化发酵条件对于最大限度地提高所需代谢物的产量至关重要.
研究的目的:
- 研究由Streptomyces sp.产生的二次代谢产物. 在不同的发酵条件下NRRL S-1813.
- 为了阐明孤立化合物的结构和形成机制,oxazolomycin A和oxazolomycin A2.
- 确定针对性生产oxazolomycin A和oxazolomycin A2.2的最佳条件.
主要方法:
- 在Streptomyces sp.的发酵过程中. 在不同介质中的NRRL S-1813.
- 隔离和净化二次代谢产物.
- 使用质谱法 (MS) 和核磁共振 (NMR) 光谱法阐明结构.
- 研究反应机制,包括非酶转化.
主要成果:
- 同时分离了氧化胺素A和氧化胺素A2.
- 鉴定出zolomycin A2 是zolomycin A 的自发性非酶环裂变的产物.
- 在性条件下,Oxazolomycin A2生产的最佳条件被确定为Medium B.
- 中等的pH值和反应时间是oxazolomycin A生物合成的关键因素,pH值略微酸性,控制时间最小化副产品.
结论:
- 这项研究成功地鉴定和表征了两种来自Streptomyces sp.的zolomycins. 在NRRL S-1813.13中.
- 阐明了一种新的非酶性转化途径,从oxazolomycin A转化为oxazolomycin A2.
- 可以采用特定的发酵策略来选择性地增强氧沙胺素A或氧沙胺素A2的生产.
关键词:
杆菌种 (Streptomyces) sp. 这种杆菌种 (Streptomyces) 是一种 在NRRL S-181313中使用.非酶环裂变的非酶环裂变奥克萨佐洛米辛A是一种奥克萨佐洛米 A2 的使用.二次代谢产物二次代谢产物更多相关视频
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