条纹生真菌劫持宿主转录抑制剂以诱导TaSWEET14d,以增强小麦糖分的吸收
Peijing Zheng1,2, Pu Yuan2, Nannan Fang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
Science advances
|July 11, 2025
概括
小麦植物使用糖最终将出口运输器 (SWEETs) 来移动糖. 发现一种特定的载体,TaSWEET14d,通过促进糖的运动来帮助条纹病原体 (Pst),突出了对疾病耐药性的新目标.
科学领域:
- 植物病原体相互作用
- 分子植物病理学 分子植物病理学
- 小麦遗传学 小麦遗传学
背景情况:
- 生物营养性病原体需要宿主碳才能生长,从而与植物竞争.
- 植物糖运输器,如糖最终将被出口运输器 (SWEETs),对于碳分配至关重要,并且是病原体的目标.
- 了解这些相互作用是培养作物抗病能力的关键.
研究的目的:
- 调查小麦SWEET家族成员TaSWEET14d在与Puccinia striiformis f. sp.的相互作用中的作用. 三子 (Pst) 的意思.
- 阐明涉及TaSWEET14d,小麦转录因子和Pst效应因子的调控机制.
- 确定增强小麦抗条纹的潜在目标.
主要方法:
- 在小麦中识别和描述TaSWEET14d.
- 基因淘汰和过度表达研究,以评估TaSWEET14d在Pst耐药性中的功能.
- 使用分子和生物化学技术分析转录因子结合和效应因子-病原体相互作用.
主要成果:
- TaSWEET14d在小麦中促进了黑和糖的转移,并由Pst.诱导.
- 失去了 TaSWEET14d 功能会增强小麦对 Pst 的抵抗力,而其过度表达会增加易感性.
- 小麦转录因子TaMYB50抑制了TaSWEET14d的表达,从而产生了耐药性.
- Pst效应物 Pst15882 与 TaMYB50 相互作用,破坏了 TaSWEET14d 的抑制,并促进了真菌入侵.
结论:
- TaSWEET14d是一个关键的宿主因子,Pst利用它来获取糖.
- Pst效应器 Pst15882采用了一种新的策略来操纵宿主基因表达,以使病原体受益.
- 准TaSWEET14d或其调节途径为在小麦中开发持久条纹抗性提供了一个有希望的途径.
更多相关视频
08:12Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum
Published on: March 11, 2020
12.2K
12:36High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
Published on: February 9, 2019
12.8K
相关概念视频
01:56Riboswitches
8.6K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.6K
01:17Gene Regulation During Sporulation
84
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
84