高温诱导雄性斑马鱼的生殖功能障碍通过DNA甲基化介导TSSK6下调调节
Xuan Ma1, Rongjian Liu1, Zhongchang Ren1
1Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Ecotoxicology and environmental safety
|July 11, 2025
概括
高温 (HT) 通过影响精子质量和丸发育,损害雄性斑马鱼的生殖能力. 丸特异性血清激酶6 (tssk6) 表达的表观遗传变化与HT诱导的生殖功能障碍有关.
科学领域:
- 环境毒理学环境毒理学
- 水生生物学 水生生物学
- 生殖生物学 生殖生物学
背景情况:
- 高温 (HT) 是水生生物的重要环境压力因素.
- 鱼类中HT诱导的生殖功能障碍的分子机制尚不清楚.
研究的目的:
- 研究暴露于HT的雄性斑马鱼生殖功能障碍的分子机制.
- 为了确定HT诱导的生殖损伤的潜在生物标志物.
主要方法:
- 雄性斑马鱼在短期 (<21天) 和长期 (>120天) 暴露于HT (33°C).
- 进行了生殖参数 (受精率,精子质量,丸发育) 的评估.
- 评估了转录组分析和基因表达 (tssk6).
- 分析了tssk6促进体区域的DNA甲基化.
主要成果:
- 暴露于HT诱导生殖功能障碍,包括降低受精率,降低精子质量,抑制丸发育和增加后代死亡率.
- 短期HT破坏了丸能量代谢;长期HT损害了精子-卵子识别.
- 丸特异性血清激酶6 (tssk6) 表达在HT下降调节.
- Tssk6-mRNA注射部分挽救了精子质量.
- 长期的HT增加了TSK6促进体中的DNA甲基化,抑制了其表达.
结论:
- 环境HT通过分子和表观遗传变化对雄性鱼的繁殖产生负面影响.
- Tssk6在缓解HT引起的生殖功能障碍方面发挥着至关重要的作用.
- Tssk6可以作为评估受热应激影响的水生生物的生殖健康的潜在生物标志物.
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