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Updated: Jul 9, 2026

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
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乳标签使得在细胞培养中实现全蛋白质组的循环动力学分析

Lorena Alamillo1, Dominic C M Ng1, Jordan Currie1

  • 1Department of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.

Cell reports methods
|July 11, 2025
PubMed
概括

一种新的重水标记方法准确地测量了各种细胞中的蛋白质循环. 这种方法揭示了人类干细胞中快速循环的关键蛋白质,有助于理解细胞过程和疾病.

关键词:
科普:分子生物学CP: 系统生物学.降级 degron 降级 降低 降低沉水是重水,重水是重水,重水是重水.这是 hiPSC 的.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量蛋白质合成 蛋白质合成蛋白质营业额的变化一些秘密一些秘密.营业额的动力学

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...