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在高流行性乳腺炎群中Streptococcus uberis的遗传多样性和分子流行病学

A Srithanasuwan¹, Y Zou², W Suriyasathaporn³

¹School of Veterinary Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand; Department of Animal Sciences, Wageningen University, 6708 PB Wageningen, the Netherlands.

Journal of dairy science

|July 11, 2025

概括

受乳腺炎影响的奶牛中Streptococcus uberis的遗传多样性因农场管理而异. 抗微生物耐药性和毒性因子是相关的,强调了针对这种具有挑战性的病原体进行量身定制的控制策略的必要性.

科学领域:

兽医微生物学兽医微生物学
牛乳腺炎研究牛乳腺炎研究
细菌基因组学细菌基因组学

背景情况:

杆菌 (Streptococcus uberis) 是牛乳腺炎的主要原因,由于其遗传变异性,这给控制带来了重大挑战.
了解S. uberis的遗传多样性,毒性因子 (VF) 和抗菌耐药性 (AMR) 对于有效的乳腺炎管理至关重要.

研究的目的:

调查三家高发病率乳腺炎农场的S. uberis分离物的遗传变异.
在农场管理实践中探索毒性因素,抗菌素耐药性和菌株多样性之间的关系.

主要方法:

全基因组测序 (WGS) 对138个S. uberis分离物进行了测序,这些分离物来自三个农场的92次感染事件.
病毒性因子 (VF) 和抗菌素耐药性 (AMR) 基因被分为核心,辅助和独特的基因组.
遗传学分析确定了7个不同的族群和32个基因模式;统计测试检查了VF-AMR相关性.

主要成果:

经验丰富的管理层的B农场表现出最高的遗传多样性,而其他农场的遗传多样性较低,模式更为主导.
发现了家族遗传/农场因素与毒性和AMR的相互作用之间存在显著的关联.
增加的AMR基因与更大的毒性多样性相关;特定的VF基因 (例如cfu,hasA) 与AMR基因有显著的相关性.

结论:

农场管理实践显著影响S. uberis的遗传多样性,毒性和抗菌素耐药性.
菌和抗菌耐药性之间的复杂关系强调了对Streptococcus uberis乳腺炎进行特定农场控制策略的需要.
针对个别乳腺炎情景的进一步研究对于制定有效的控制措施至关重要.

关键词:

Streptococcus uberis 是一个细菌.抗微生物耐药性抗微生物耐药性乳腺炎是一种乳腺炎.毒性因子是毒性因子.

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