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在DevTox Germ Layer Reporter平台中选工业化学品对人类发育的毒性

John T Gamble¹, Jesse Rogers², Kristen Breaux²

¹Center for Computational Toxicology and Exposure, Office of Research and Development, US. Environmental Protection Agency, Research Triangle Park, NC 27711, United States; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN 37831, United States.

|July 11, 2025

概括

德沃克斯细菌层报告器 (GLR) 试验有效地选化学物质的发育毒性. 这种新方法方法 (NAM) 帮助环保署评估新化学品提交对孕妇和发育中的后代的潜在风险.

科学领域:

毒理学毒理学毒理学
发展生物学发展生物学
化学安全评估化学安全评估

背景情况:

有毒物质控制法 (TSCA) 要求对新化学品进行有效审查.
现有的发展性毒性选方法不足以快速识别危害.
新方法方法 (NAMs) 为化学安全评估提供了有前途的替代方案.

研究的目的:

通过使用DevTox细菌层报告器 (GLR) - Endo测试来选各种化学物质.
评估测试对发育性毒素的性能和预测性.
评估与美国环保署新化学品合作研究计划 (NCCRP) 相关的化学品.

主要方法:

使用DevTox GLR-Endo测试对171种TSCA化学物质和54种体外测试参考化学物质进行选.
对16种参考发育毒性物质的测试性能指标和预测能力的评估.
对特定测试终点 (如SOX17) 的化学活性和选择性活性分析.

主要成果:

德沃克斯GLR-Endo测试显示了可接受的性能和预测性.
在之前未评估的38种参考化学物质中,有25种是活性化学物质,其中13种具有选择性活性.
在165种测试的TSCA化学品中,有60种是活性化学品,29种具有选择性活性.

结论:

DevTox GLR-Endo测定是一种可行的工具,用于快速选发育性毒性.
结果支持开发基于NAM的新化学品危险评估策略.
这项研究为NCCRP相关的化学品提供了关键的毒理数据.

关键词:

发展性毒性发展性毒性高通量选的高通量选新方法新方法的方法.新化学品合作研究项目合作研究项目有毒物质控制法案是有毒物质控制法案.

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