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在急性热应激下,狭爪鱼 (Pontastacus leptodactylus) 的生理反应和组织病理变化

Xia Zhu^1,2,3, Bin Li⁴, Yuzhen Liu^1,2,3

¹Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China.

Animals : an open access journal from MDPI

|July 12, 2025

概括

急性热应激会损害窄爪 (Pontastacus leptodactylus),导致肝损伤和25°C以上的死亡率. 这项研究通过确定生存的临界温度值来指导水产养殖实践.

科学领域:

水生物质毒理学研究
甲动物生理学甲动物生理学
分子生物学分子生物学

背景情况:

窄爪 (Pontastacus leptodactylus) 是一个重要的水产养殖物种.
了解它们的耐热性对于可持续的水产养殖至关重要.

研究的目的:

研究急性热应激对P. leptodactylus的影响.
为了确定生理和分子对高温的反应.
为了确定P. leptodactylus水产养殖的安全温度限制.

主要方法:

将P. leptodactylus暴露在15°C,20°C,25°C和30°C的温度中,长达72小时.
评估生存率,血淋巴生物化学,抗氧化能力和肝胰腺组织病理学.
对肝胰腺组织进行比较性转录分析.

主要成果:

在48小时后,在30°C组观察到显著的死亡率.
肝胰腺组织损伤,包括亡和结构变化,发生在25°C和30°C.
在更高的温度下,观察到高反应性氧物种 (ROS),改变抗氧化酶活性 (SOD,CAT) 和降低总抗氧化能力 (T-AOC).
转录组分析显示,在热应激下,代谢和亡途径的丰富.

结论:

急性热应激会通过肝胰腺损伤,代谢中断和ROS积累诱导P. leptodactylus的死亡.
温度超过25°C对P. leptodactylus的生存有害.
这些发现为优化P. leptodactylus水产养殖管理和防止库存损失提供了关键的见解.

关键词:

在RNA-seqqq.能量代谢能量代谢狭爪鱼是一种狭爪鱼.热应力是一种热应力.

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