相关实验视频
Updated: Sep 16, 2025

10:24
Xenopus laevis as a Model to Identify Translation Impairment
Published on: September 27, 2015
10.8K
LncRNA RWDD3 通过调节 miR-1388-5p/NPY1R/cAMP 途径,促进莱迪格细胞的类固醇生成
Meijing Chen1, Xuejiao Yin2,3, Chunhui Duan1
1College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Animals : an open access journal from MDPI
|July 12, 2025
概括
益生菌通过影响喀什米尔山羊的激素分泌来调节雄性生殖. 一种新的长非编码RNA, lncRWDD3,作为ceRNA通过miR-1388-5p/NPY1R通路来增强这个过程.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- 益生菌对男性生殖至关重要,但其对分泌的调节以及长非编码RNAs (lncRNAs) 的作用尚未完全理解.
- 研究这些机制对于了解男性生殖健康至关重要.
研究的目的:
- 阐明益生菌通过什么分子机制调节喀什米尔山羊莱迪格细胞中的丸激素合成.
- 识别和描述参与前素中介的新型lncRNAs的作用.
主要方法:
- 来自喀什米尔山羊的丸组织的RNA测序,具有不同水平的益生菌素.
- 构建一个lncRNA-miRNA-mRNA相互作用网络.
- 在体外实验中使用初级莱迪格细胞研究lncRWDD3,miR-1388-5p和NPY1R的功能.
主要成果:
- 200 ng/mL的普罗拉克丁最大限度地刺激了莱迪格细胞中丸激素的分泌.
- 过度表达lncRWDD3或NPY1R增加了cAMP水平和激素分泌,而干扰则产生了相反的效果.
- lncRWDD3作为对 miR-1388-5p 的竞争性内源RNA (ceRNA) 起作用,该RNA针对NPY1R.
结论:
- 益生菌通过lncRNA-miRNA-mRNA ceRNA网络调节喀什米尔山羊的丸功能.
- lncRWDD3通过菌miR-1388-5p激活NPY1R/cAMP信号通路,从而调节莱迪格细胞中的合成.
相关概念视频
lncRNA - Long Non-coding RNAs
9.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
Formation of Muscle Fibers from Myoblasts
5.2K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.2K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Regulation of Expression at Multiple Steps
1.0K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.0K
Regulation of Expression Occurs at Multiple Steps
23.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
23.5K
Master Transcription Regulators
7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K

