在整个养殖和加工过程中研究Hermetia illucens粉末的微生物动力学:使用文化和元编码方法的综合方法
Boris Misery1, Lenaïg Brulé1, Rima Djema1
1Oniris, INRAE, SECALIM, 44300 Nantes, France.
Foods (Basel, Switzerland)
|July 12, 2025
概括
处理黑士兵 (BSF) 幼虫对微生物安全有影响. 沸后干燥显著降低了细菌负载,但仍然存在一些子形成细菌和坎皮洛巴克特,需要对这种可持续蛋白质来源进行进一步的安全评估.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 可持续农业 可持续农业
背景情况:
- 对于可持续蛋白质来源的日益增长的需求将黑士兵 (BSF) 幼虫定位为可行的替代品.
- 微生物安全是BSF幼虫在食品和料中广泛采用的关键问题.
- 了解BSF幼虫的微生物特征和加工的影响对于确保安全至关重要.
研究的目的:
- 为了研究BSF幼虫的微生物多样性.
- 为了比较两种加工方法 (沸然后干燥与单独干燥) 对微生物负荷的有效性.
- 评估 BSF幼虫在潜在的食品应用中的微生物安全性.
主要方法:
- 使用16S rRNA测序来分析微生物多样性.
- 基于培养的方法量化了细菌负载 (中性细菌,子,乳酸细菌,酵母,模具,硫酸盐减少细菌).
- 一项系统性审查综合了关于BSF微生物群的现有知识.
主要成果:
- 在基质和幼虫中观察到高初始负载的中性细菌,子和乳酸细菌.
- 沸后干燥显著减少了大多数微生物负载,包括酵母,菌和无氧硫酸盐减少细菌.
- 虽然关键的病原体不存在,但Campylobacter sp. 在粉末中检测值得注意食品安全.
结论:
- 与单独干燥相比,煮沸和干燥在减少BSF幼虫中的微生物负荷方面更有效.
- 其余的子形成细菌 (Bacillus,Clostridium) 和Campylobacter的存在凸显了优化加工的必要性.
- 需要进一步的研究和严格的安全协议才能充分实现BSF幼虫作为可持续蛋白质的潜力.
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