在Lactiplantibacillus plantarumB22中剖析高酶/脂酶活性和益生菌特征:基因组引导的功能性表征
Yunmei Chai1, Zhenzhu Li1, Wentao Zheng1
1Department of Food Science, College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Foods (Basel, Switzerland)
|July 12, 2025
概括
乳植物菌植物B22显示出高的脂酶活性和益生菌潜力. 基因组分析证实了其安全性,并确定了脂质代谢的关键基因,支持其作为功能益生菌的发展.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 益生菌 益生菌 益生菌
背景情况:
- 乳植物菌植物B22具有高的酶/脂酶活性,但其完整的基因组和益生菌能力尚不清楚.
- 了解其脂解活性和益生菌特征的遗传基础对于其应用至关重要.
研究的目的:
- 为了研究高脂解活性和益生菌潜力的基因组和功能基础的Lactiplantibacillus plantarum B22.
- 描述菌株的安全性,并确定与脂质代谢和益生菌功能相关的基因.
主要方法:
- 全基因组测序和L. plantarum B22的注释.
- 分子对接研究来分析酶-基质相互作用.
- 现型测定用于评估胃肠道耐受性,抗氧化剂和抗菌活性.
主要成果:
- L. plantarum B22 (2,027,325 bp) 的完整基因组编码了2005个基因,包括13个酶/脂酶基因,其中4个具有必要的催化三合体.
- 该菌株表现出强烈的脂酶活性 (3.45 ± 0.13 U/mL) 和显著的结合亲和力与tributyrin.
- L. plantarum B22对模拟的胃肠道状况,强大的抗氧化和抗菌作用表现出极好的耐受性,并证实安全,没有发现的毒性因子或有害代谢物.
结论:
- L. plantarum B22具有显著的脂解活性和强大的益生菌潜力,由其基因组和表型特征支持.
- 已识别的酶/脂酶基因和脂质代谢途径为了解其功能能力提供了基础.
- 安全性概况和证明的益生菌特征使L. plantarum B22成为功能益生菌开发的有希望的候选者.
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