相关实验视频

Updated: Jun 20, 2026

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

使用在乳腺甲状腺小癌中的循环等离子体miRNAs进行攻击性的诊断和评估

Jiwon Jang¹, Ji Min Kim¹, Sung-Chan Shin¹

¹Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Pusan National University and Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Republic of Korea.

|July 12, 2025

概括

血中六个microRNA的小组可以帮助区分乳头甲状腺小癌 (PTMC) 和良性结节,并预测瘤的攻击性. 这一发现有可能改善甲状腺癌研究中的PTMC诊断和风险分层.

科学领域:

生物标志物生物标志物
分子诊断学分子诊断
在瘤学瘤学.

背景情况:

微RNAs (miRNAs) 显示为各种疾病的诊断标记物,包括乳头甲状腺癌 (PTC).
有限的研究存在于循环miRNA表达,特别是在乳头甲状腺微癌 (PTMC).
血衍生的miRNAs需要研究它们的潜力,以区分PTMC和良性结节,并评估瘤的攻击性.

研究的目的:

在患有PTMC的患者中研究血衍生的miRNA表达特征.
为了确定循环中的miRNA是否可以区分PTMC与良性甲状腺结节.
评估miRNAs在预测PTMC的攻击性方面的潜力.

主要方法:

150名接受甲状腺切除术的患者被分为良性,低风险PTMC和高级PTMC组.
在每组9名患者中,使用微阵列分析分析了血miRNA表达.
使用TaqMan试验进一步验证了六个选定的miRNA,并对诊断和预后价值进行了ROC曲线分析.

主要成果:

微阵列分析确定了miR-455-3p和miR-548ac作为良性结节和PTMC之间的关键差异.
六种miRNAs (miR-455-3p,miR-548ac,miR-221,miR-222,miR-146a,miR-146b) 的组合实现了良性与PTMC的高诊断性能 (AUC 0.857).
六个miRNA组合还显示出在区分低风险PTMC和攻击性PTMC (AUC0.763) 中的潜力.

结论:

六种血miRNAs的特定组合可以有效地区分良性甲状腺结节与PTMC.
这种miRNA面板在区分低风险和攻击性PTMC方面也显示出希望.
这些发现表明,这六个miRNA签名在PTMC的非侵入性诊断和风险分层中可能发挥作用.

关键词:

攻击性攻击性攻击性的miRNA表达水平.这是一个微型RNA.甲状腺小瘤是甲状腺小瘤.

更多相关视频

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...