在EDTA等离子体中评估D-dimer测量的可靠性:与既定的酸盐方法进行比较
Daniel Pfingst1,2, Adriana Méndez1, Peter Neyer1
1Institute of Laboratory Medicine, Cantonal Hospital Aarau, 5001 Aarau, Switzerland.
Diagnostics (Basel, Switzerland)
|July 12, 2025
概括
乙烯基二胺四酸 (EDTA) 血是用于D-二元体检测的酸血的可行替代品,为静脉血栓塞栓症风险评估提供类似的诊断潜力.
科学领域:
- 临床化学 临床化学
- 血液学 血液学 血液学
- 生物标记分析 生物标记分析
背景情况:
- 酸血中的D-二聚体是排除静脉血栓塞栓症 (VTE) 的关键生物标志物.
- 在某些临床场景中,酸血的可用性可能是局限性的.
- 调查替代样品类型对于更广泛的D-二次元实用性至关重要.
研究的目的:
- 评估使用乙烯基二胺三酸 (EDTA) 血用于D-二分体测量的可行性.
- 为了比较从EDTA血获得的D-二聚体结果与传统的酸血.
- 为了评估不同等离子体矩阵中的D-二分体测试的诊断等价性.
主要方法:
- 匿名血样本 (n=99) 用酸盐和EDTA样本进行分析.
- 采用了INNOVANCE® D-dimer测定和INNOVANCE® LOCI hs D-dimer测定. 这两种测定都是使用的.
- 在酸盐和EDTA血中的D-二聚物测量之间进行了相关性分析.
主要成果:
- 在化血中观察到高相关性 (r ≥ 0.97) 的D-二聚合物测定.
- 在调整为酸盐稀释后,在酸盐和EDTA血之间保持了强烈的相关性 (r ≥ 0.96).
- 结果表明这两种血类型之间的D-二聚物水平可比.
结论:
- 乙烯基二胺四酸 (EDTA) 血是一种可行的替代D-二分体测试.
- 虽然可行,但观察到的差异可能会影响临床解释和风险分层.
- 需要进一步的研究来验证这些发现,并确定EDTA血的具体切断值.
相关概念视频
01:18Effects of EDTA on End-Point Detection Methods
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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
350
01:23EDTA: Indirect and Alkalimetric Titration
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Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
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01:22EDTA: Chemistry and Properties
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Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
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01:20Complexometric EDTA Titration Curves
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EDTA titration curves determine the free metal ion concentration. The titration curve represents the change in concentration of free metal ions (p function) as a function of the volume of EDTA added. This curve consists of three regions: before, at, and after equivalence points. Excess free metal ions are present before the equivalence point. Equal concentrations of metal ions and EDTA are present at the equivalence point. After the equivalence point, excess EDTA exists. This means slight...
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01:30EDTA: Direct, Back-, and Displacement Titration
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The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
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01:26EDTA: Auxiliary Complexing Reagents
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EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
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