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Updated: Sep 16, 2025

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
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基于原生质的再生使CRISPR/Cas9在两种温度Japonica的水作物中应用成为可能

Marion Barrera1, Blanca Olmedo1, Matías Narváez2

  • 1Biotechnology Laboratory, La Platina Research Station, National Institute of Agriculture (INIA), Santiago 8831314, Chile.

Plants (Basel, Switzerland)
|July 12, 2025
PubMed
概括

一个新的协议使得温带气候的日本大米中的原质细胞再生和基因编辑成为可能. 这一进步支持利用新基因组技术 (NGT) 提高作物适应性和弹性.

关键词:
智利大米 智利大米酸封装封装的阿尔吉纳特适应寒冷的适应性大米料提取物 料提取物新的基因组技术.转化过程中的转化.

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CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Homologous Recombination

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Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
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