通过实时质子质谱法对淋巴管状瘤的呼气分析
Malika Mustafina1,2,3, Artemiy Silantyev4, Marina Makarova2,5
1Department of Cardiology, Functional and Ultrasound Diagnostics, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia.
International journal of molecular sciences
|July 12, 2025
概括
使用质子转移反应质谱法 (PTR-MS) 进行的呼气分析显示,对诊断淋巴血管菌病 (LAM) 有希望. 这项研究确定了呼吸中的挥发性有机化合物 (VOC),可以检测LAM并预测其并发症.
科学领域:
- 肺部医学 肺部医学
- 生物标志物发现发现
- 分析化学 分析化学
背景情况:
- 淋巴细胞结核病 (Lymphangioleiomyomatosis,简称LAM) 是一种罕见的,在生育年龄的女性中进展性肺部疾病.
- 目前的LAM诊断是昂贵的或缺乏特异性,需要新的非侵入性方法.
- 呼气分析为识别特定疾病的挥发性有机化合物 (VOC) 提供了潜在的途径.
研究的目的:
- 为了调查呼出的气息挥发性有机化合物 (VOC) 档案,以诊断LAM.
- 为了确定可预测LAM并发症的VOC生物标志物.
- 为了评估LAM的VOC标志的诊断准确性.
主要方法:
- 一项涉及51名LAM患者和51名健康对照者的横截面研究.
- 实时质子转移反应飞行时间质谱 (PTR-TOF-MS) 用于呼气分析.
- 机器学习模型,包括XGBoost,用于特征选择和分类.
主要成果:
- 确定了潜在的LAM生物标志物:乳酸 (m/z = 90.06) 和 m/z = 113.13.3.
- 已识别的可预测并发症的VOCs:甲乙醇 (m/z = 49.00),O-甲基胺 (m/z = 48.04) 和m/z = 129.07.
- 使用具有已识别生物标记物的分类器,实现了高诊断准确性 (AUC = 0.922).
结论:
- 呼出呼吸中的VOC分析是一个有前途的非侵入性工具,用于LAM诊断.
- 挥发性有机化合物签名可以预测LAM并发症,如肺胸部和通风障碍.
- 为了完善临床应用,需要在更大的队列中进一步验证.
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