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人类外周血液单核细胞暴露于50赫兹磁场的蛋白质特性

Massimo Bracci¹, Raffaella Lazzarini¹, Francesco Piva²

¹Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy.

International journal of molecular sciences

|July 12, 2025

概括

极低频磁场 (ELF-MF) 改变人类细胞代谢和免疫反应. 暴露于ELF-MF可以调高线粒体蛋白质,并降低外周血液单核细胞 (PBMC) 中的免疫相关蛋白质,而不会影响细胞活力.

科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
蛋白质组学是指蛋白质组学.

背景情况:

众所周知,极低频磁场 (ELF-MF) 在人类细胞中诱导生物效应.
细胞对ELF-MF反应的分子机制和调节因素,特别是外围血液单核细胞 (PBMCs) 中的反应,尚不清楚.

研究的目的:

调查暴露于ELF-MF后人类PBMC中的蛋白质变化.
为了确定受PBMC中ELF-MF暴露影响的关键蛋白质和分子通路.

主要方法:

来自三个暴露于50 Hz,1 mT ELF-MF24小时的人的PBMCs的蛋白质基因分析.
从同一个人中暴露和未暴露的PBMC之间的蛋白质表达水平的比较.

主要成果:

暴露于ELF-MF改变了PBMC蛋白表达,但没有影响细胞增殖,活力或细胞周期.
51种蛋白质被上调,其中36种与细胞和代谢过程有关,包括SLC25A4 (线粒体ATP/ADP交换).
67种蛋白质的下调,许多与T细胞共刺激,细胞激活和免疫系统过程有关.

结论:

暴露于ELF-MF诱导了人类PBMC中的代谢重编程.
观察到的变化包括线粒体蛋白的上调和免疫激活相关蛋白的下调.
在测试条件下,细胞活力和增殖不受ELF-MF暴露的影响.

关键词:

在ELF-MFF中.这就是PBMCs.免疫功能免疫功能免疫功能代谢途径代谢途径线粒体的ADP/ATP运输方式蛋白质组的个人资料蛋白质组的个人资料

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