肌肉发育中的circhomer1的功能和分子机制
Zonggang Yu1, Kaiming Wang1, Bohe Chen1
1College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
International journal of molecular sciences
|July 12, 2025
概括
循环RNA HOMER1 (circHOMER1) 促进骨肌细胞的增殖和亡,同时抑制宁翔猪的分化. 这一发现为肌肉发育和潜在的育种策略提供了洞察力.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 动物科学动物科学
背景情况:
- 骨肌肉发育对于肉质量和理解肌肉疾病至关重要.
- 循环RNA (circRNAs) 在生物过程中发挥作用,但它们在骨肌肉中的功能需要进一步研究.
- circHOMER1在肌肉细胞发育中的特定作用尚不清楚.
研究的目的:
- 调查宁翔猪骨肌肉中circHOMER1的真实性,稳定性和表达模式.
- 确定 circHOMER1 对肌细胞增殖,细胞亡和分化的功能影响.
- 阐明 circHOMER1 在肌肉细胞发育中的作用背后的分子机制.
主要方法:
- 使用分子技术检测circhHOMER1的真实性和稳定性.
- 在脂肪组织和肌细胞中分析circhHOMER1的时空表达.
- 功能性测试涉及circHOMER1过度表达和干扰肌细胞,评估增殖,亡和分化标志物.
- 亚细胞局部化和分子机制分析,包括相互作用网络和潜在的编码.
主要成果:
- circHOMER1在宁夏猪骨肌肉中得到证实,表现出比线性RNA更大的稳定性和脂肪组织和早期肌肉细胞生长中的上调.
- 通过标记基因/蛋白质表达和细胞周期分析, circHOMER1 的过度表达促进了肌细胞增殖和亡,同时抑制了分化.
- 机制分析表明circHOMER1的细胞质局部化及其参与一个复杂的调节网络与miRNAs,lncRNAs和mRNAs,可能编码小.
结论:
- circHOMER1显著影响宁翔猪的肌细胞增殖,亡和分化.
- circHOMER1可能通过ceRNA机制或编码小来调节肌细胞细胞的发育.
- 这些发现为了解肌肉发育调节提供了基础,并为宁翔猪的育种策略提供了信息.
相关概念视频
02:23Master Transcription Regulators
7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K
01:13Formation of Muscle Fibers from Myoblasts
5.2K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.2K
02:15M-Cdk Drives Transition Into Mitosis
5.7K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.7K
02:39Positive Regulator Molecules
5.7K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.7K
02:19Circadian Rhythms and Gene Regulation
4.2K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.2K
01:57Protein Complexes with Interchangeable Parts
2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K