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短期益生菌殖民改变3D口服生物膜的分子动力学

Nadeeka S Udawatte¹, Chun Liu¹, Reuben Staples¹

¹Center for Oral-facial Regeneration, Rehabilitation and Reconstruction (COR3), School of Dentistry, The University of Queensland, Brisbane, QLD 4006, Australia.

International journal of molecular sciences

|July 12, 2025

概括

口服益生菌Streptococcus salivarius K12 (Ssk12) 在3D口服生物膜模型中显示了长达7天的短暂殖民. 短期的Ssk12使用改变了微生物群落,提高了抗压能力,表明功能转变的潜力.

科学领域:

口腔微生物学口腔微生物学
生物膜研究的研究.
益生菌科学是一种益生菌科学.

背景情况:

三维 (3D) 支架系统对于研究口服多微生物生物膜和益生菌相互作用至关重要.
Salivary Streptococcus K12 (Ssk12) 是一种有前途的口服益生菌,可以调节微生物平衡.
了解Ssk12的附着性,持久性和整合机制对于其治疗疗效至关重要,但仍然不太了解.

研究的目的:

在口服生物膜上研究Ssk12的时间殖民动态.
评估Ssk12殖民对唾液生物膜结构和功能的影响.
通过使用3D支架,探索Ssk12与本地口腔微生物组的整合.

主要方法:

使用融电写聚-ε-caprolactone (MEW-mPCL) 支架来模仿口腔的利基.
使用smFISH,共聚焦显微镜和RT-qPCR监测Ssk12殖民.
通过16S rRNA测序和meta-transcriptomics评估生物膜社区的转移和功能.

主要成果:

Ssk12显示过渡殖民,可检测长达7天,在第10天减少.
药物后观察到乳杆菌和双菌种群的短期增加.
功能分析揭示了氧化应激抵抗和代谢适应的增强的转录特征.

结论:

即使是Ssk12的短期殖民也可以诱导口腔生物膜的显著功能变化.
Ssk12殖民的短暂性质凸显了提高其持久性的战略的需要.
需要进一步的研究来优化益生菌策略,以获得持续的口腔健康益处.

关键词:

3D化电写 (MEW) 医疗级的聚烯酸 (mPCL)3D唾液多微生物生物膜模型口腔的微生物群.益生菌殖民化的殖民化杆菌液杆菌K12 (Ssk12) 是一种

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