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跨代表观遗传和表型遗传在绵羊中跨越五代
Mehmet Kizilaslan1, Camila U Braz2, Jessica Townsend1
1Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
International journal of molecular sciences
|July 12, 2025
概括
在绵羊父亲 (F0) 中补充氨酸导致其后代 (F3和F4) 具有持久的跨代表观遗传特征. 这种饮食干预影响了F2代以外的后代表型.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 畜牧科学 畜牧科学 畜牧科学
- 营养基因组学 营养基因组学
背景情况:
- 表观遗传信息可以在几代人之间继承,尽管在发育过程中进行了重编程.
- 现象型的跨代表观遗传 (TEI) 是至关重要的,但研究不足,特别是在牲畜的饮食干预方面.
- 很少有研究研究了营养干预对农场动物表观遗传的长期影响.
研究的目的:
- 调查甲胺补充剂对绵羊表观遗传修饰和表型的跨代影响.
- 识别不同甲基基因 (DMG),在多代 (F3和F4) 中表现出遗传性.
- 为了将饮食干预效应与遗传的表观遗传变化和后代表型相关联.
主要方法:
- 全基因组二硫酸盐测序 (WGBS) 用于单基底分辨率差异甲基化分析.
- 分析F3和F4代的差异甲基化细胞因子 (DMC) 和差异甲基化基因 (DMG).
- 对照和补充 metionin的F0双对雄羊及其后代的表观遗传数据的比较.
- 估计F0饮食对F3和F4生长和生育表型的影响大小.
主要成果:
- 确定了41个跨代表观遗传 (TEI-DMG) 的DMG,跨四代,以及11个跨五代的TEI-DMG.
- 检测到对照品种和补充 metionin 的品种之间甲基化模式的显著差异.
- 证明了F0饮食组,遗传的不同甲基化基因,以及F3和F4世代的后代生长和生育表型之间的相关性.
- 提供了超过F2世代的饮食干预的长期跨代影响的证据.
结论:
- 适度的饮食干预 (甲胺补充剂) 可以诱导绵羊表型的持续跨代表观遗传.
- 这项研究提供了第一个基因水平和表型证据,证明长期的饮食对绵羊F2代以外的后代表型的影响.
- 氨酸补充对表观遗传标记和随后的表型的影响强调了营养策略在几代人之间影响牲畜健康和生产力的潜力.
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