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在Achnatherum splendens中AsSWEET基因家族的识别和表达模式分析

Ming Hu¹, Wei Kou², Mingsu Chen¹

¹Key Laboratory of Oasis Town and Mountain-Basin System Ecology, Xinjiang Production and Construction Corps, Key Laboratory of Xinjiang Phytomedicine Resource Utilization, Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China.

International journal of molecular sciences

|July 12, 2025

概括

这项研究在Achnatherum splendens中确定了31种最终将出口的糖载体 (SWEET),揭示了参与盐和干旱压力反应的关键基因. 这些AsSWEET提供了对植物抗压力机制的见解.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

糖最终将被出口运输 (SWEETs) 对植物生长和环境压力耐受性至关重要.
阿克纳瑟 (Achnatherum splendens) 具有显著的盐和干旱耐受性,使其成为研究抗压力的模型.

研究的目的:

为了识别和描述Achnatherum splendens中的SWEET基因家族.
研究AsSWEET基因在盐和干旱压力反应中的潜在作用.

主要方法:

来自Achnatherum splendens数据库的31个AsSWEET基因的全基因识别.
生物信息分析包括基因结构,定位,促进元件,系系和染色体分布.
定量实时PCR (qRT-PCR) 在压力条件下分析基因表达.

主要成果:

确定了31个AsSWEET基因,分布在13个染色体中,具有不同的预测长和物理化学特性.
促进体分析显示,所有AsSWEET基因中的光,压力和激素反应相关的cis作用元素.
亚细胞局部化预测表明不同AsSWEETs的等离子体膜,质体,质体和核局部化.
qRT-PCR证实了在盐和干旱压力下对A. splendens根的AsSWEET13-1,AsSWEET13-3和AsSWEET1a的调节.

结论:

已识别的AsSWEET基因具有特征,表明它们在植物耐压力中起作用.
特定的AsSWEET基因 (AsSWEET13-1,AsSWEET13-3,AsSWEET1a) 是进一步研究A. splendens.盐和干旱抵抗机制的有希望的候选人.

关键词:

阿克纳瑟 (Achnatherum splendens) 是一个丽的植物.无生物压力是无生物压力.基因表达的基因表达方式基因家族基因家族基因家族糖运输公司糖运输公司

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