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在个性化医学中释放球状体的潜力:对播种方法的系统审查
Karolina M Lonkwic1,2, Radosław Zajdel3,4, Krzysztof Kaczka1
1Clinic of General and Oncological Surgery, Medical University of Lodz, 92-213 Lodz, Poland.
International journal of molecular sciences
|July 12, 2025
概括
三维 (3D) 癌症球体为药物查提供了更好的体外模型. 本综述分析了生成这些患者衍生模型的方法,强调了提高癌症研究可重复性的标准化需求.
科学领域:
- 在瘤学瘤学.
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
背景情况:
- 传统的二维 (2D) 细胞培养在癌症研究中缺乏生理相关性.
- 三维 (3D) 球形模型提供了更准确的体外环境.
- 来自患者的癌症球体越来越多地用于药物查.
研究的目的:
- 系统地审查和评估产生患者衍生的癌症球体的方法.
- 为了确定常见的癌症类型和用于研究的球形形成技术.
- 突出了球形培养方法的标准化需求.
主要方法:
- 系统的文学搜索对患者衍生癌症球体的英语研究,用于药物查.
- 确定了研究选择的纳入和排除标准.
- 提取有关癌症类型,培养方法,球形特征和治疗反应的数据.
主要成果:
- 乳腺,结肠,肺,卵巢和脑癌是球形模型最受研究的来源.
- 强迫浮动和基于脚手架的方法是占主导地位的球形生成技术.
- 微流体,生物打印,悬浮滴和悬浮培养物也被利用,具有不同的优势.
结论:
- 3D球形模型正在彻底改变体外癌症研究.
- 球形形成和培养方法的标准化对于提高可再生性至关重要.
- 改进标准化将增加球形模型在药物开发和癌症治疗中的翻译潜力.
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