可持续的微提取使用可切换溶解溶剂,用于尿样中的三种profenoid药物的液体色谱测定
Christina Patakidou1,2, Marianna Ntorkou1, Constantinos K Zacharis1
1Laboratory of Pharmaceutical Analysis, Department of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Journal of separation science
|July 12, 2025
概括
一种新的可切换溶剂方法有效地利用pH触发的相位过渡从尿液中提取类药物. 这种绿色分析方法为制药分析提供了精确可靠的量化方法.
科学领域:
- 分析化学 分析化学
- 环境化学环境化学
背景情况:
- 在生物样本中精确量化类药物对于治疗药物监测和药理动力学研究至关重要.
- 传统的提取方法可能耗时,并且涉及危险的有机溶剂.
研究的目的:
- 开发一种绿色和高效的微提取方法,用于在人体尿液中的类药物 (基托,芬,弗鲁比).
- 为了研究使用可切换的可溶性溶剂,以酸为基础,用于样品制备.
主要方法:
- 微提取利用盐酸作为可切换溶剂的依赖pH的相位过渡.
- 通过用酸调整尿液pH值来实现分散和相位分离.
- 使用C18列与渐变化分析提取的分析物.
主要成果:
- 该方法表现出极好的线性 (50-3000 ng/mL) 和良好的精度 (%RSD < 14.3%).
- 实现了令人满意的日内和日间准确性 (82.3%-110.1%).
- 该方法的绿色潜力通过使用多种评估工具进行了评估.
结论:
- 开发的可切换溶剂微提取方法对分析人类尿液中的类药物有效.
- 这种方法为传统的提取技术提供了一个更绿色的替代方案.
- 该方法成功地应用于托后的真实尿液样本.
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