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Updated: Sep 16, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
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通过SpCas12f1对顺序dSDNA裂变的结构和机制见解

Julene Madariaga-Marcos1, Marius Baltramonaitis2, Selgar Henkel-Heinecke1

  • 1Peter Debye Institute for Soft Matter Physics, Universität Leipzig, Leipzig 04103, Germany.

Nucleic acids research
|July 12, 2025
PubMed
概括

微型CRISPR-Cas12f1 (SpCas12f1) 基因组工程工具形成稳定的R循环. DNA 裂变涉及快速的非目标链降解,其次是较慢的目标链切割.

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