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番茄中的AtBES1D过度表达增强了BR反应,并加速了水果的成熟

Sen He¹, Xiubo Xia², Junhui Yang¹

¹College of Plant Science, Jilin University, Changchun, 130062, China.

Journal of plant physiology

|July 12, 2025

概括

在番茄中过度表达一个关键的类固醇基因,加速了水果的成熟,改变了植物的发育. 这项研究揭示了BES1.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

铜类固醇 (BRs) 是关键的植物激素,调节生长和发育.
BES1和BZR1是BR信号传输中的中心转录因子.
在番茄果实成熟过程中BES1的具体作用尚不清楚.

研究的目的:

调查阿拉比多普西斯塔利亚纳BES1D (AtBES1D) 在调节番茄生长和果实成熟方面的功能.
阐明AtBES1D影响番茄发育和水果质量的分子机制.

主要方法:

产生了三个独立的转基因番茄系,过度表达AtBES1D.
转基因植物和水果的表型分析.
乙烯相关和成熟调节基因的基因表达分析.
染色体免疫沉测序 (ChIP-seq) 用于识别AtBES1D的目标基因.

主要成果:

在BES1D中,过度表达增强了BR反应,并降低了对BR生物合成抑制剂的敏感性.
转基因植物的生长迟缓,花期延迟,果实成熟速度加快,果实尺寸更小,种子更少.
在转基因果实中观察到与乙烯相关的基因和关键成熟调节剂 (RIN,TAGL1,FUL1,FUL2,PG) 的升级表达.
ChIP-seq确定了1757个AtBES1D向基因,这些基因参与激素信号传递,转录调节和新陈代谢.

结论:

在番茄中,AtBES1D充当多功能调节剂,影响植物生长,开花时间和果实成熟.
AtBES1D通过调节BR信号,乙烯通路,转录因子和代谢过程,促进番茄果实成熟,并可能改善果实质量.

关键词:

转录因子 BES1 的转录因子.黄铜类固醇是一种类固醇.果实成熟的时间茄子番茄子番茄子

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