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一种无标签的光极化技术,用于检测食品添加剂

Shanshan Fan¹, Jilin Wang¹, Xueyi Wen²

¹Zhanjiang Central Hospital, Guangdong Medical University, Zhanjiang, 524045, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|July 12, 2025

概括

一种新的无标签测定方法使用纳米颗粒和纳米片用于同时检测葡萄糖,胆和乳酸盐. 这种方法为分析这些关键的食品添加剂提供了灵敏和高效的方法.

科学领域:

分析化学分析化学
生物材料科学生物材料科学
纳米技术纳米技术

背景情况:

准确检测葡萄糖,胆和乳酸在食品分析中至关重要.
现有的检测方法通常需要复杂的程序,标记的试剂或特定的生物分子,如抗体.
需要更简单,更有效,更无标签的分析技术.

研究的目的:

开发一种新的,无标签的光极化 (FP) 试验,用于同时检测葡萄糖,胆和乳酸盐.
为了利用牛血清蛋白纳米颗粒 (BSA NPs) 和二氧化纳米片 (MnO2 NS) 作为检测平台的关键组件.
建立一种灵敏可靠的方法,在相关度范围内量化这些分析物.

主要方法:

使用BSA NPs和MnO2 NS.设计了一种无标签的光极化 (FP) 试验.
我们利用了BSA NP和MnO2 NS之间的相互作用,其中MnO2 NS放大了BSA NP的FP信号.
由氧化酶作用于葡萄糖,胆和乳酸而产生的过氧化 (H2O2) 用于灭放大FP信号.

主要成果:

开发的BSA NPs-MnO2 NS系统在存在H2O2.2.时显示FP信号发生显著变化.
该试验证明了对葡萄糖,胆和乳酸的同时检测能力.
该方法的检测范围为葡萄糖的0-200μM,胆的0-800μM,乳酸的0-200μM.

结论:

一种新的,无抗体和无染料的无标签FP试验成功开发,用于同时检测葡萄糖,胆和乳酸盐.
BSA NPs和MnO2 NS的组合提供了一个敏感的平台来检测产生H2O2.2.的分析物.
这种方法为快速有效地分析这些食品添加剂提供了有希望的方法.

关键词:

牛血清蛋白纳米颗粒牛血清蛋白纳米颗粒光的两极化是因为光.食品添加剂食品添加剂没有标签的无标签.

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