在Bovinae亚家族内源性逆转录病毒 (ERVs) 的演变
Ahmed A Saleh1, Naisu Yang2, Amr M A Rashad3
1College of Animal Science & Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China; Animal and Fish Production Department, Faculty of Agriculture (Al-Shatby), Alexandria University, Alexandria City 11865, Egypt.
Gene
|July 12, 2025
概括
这项研究表明,内源逆转录病毒 (ERV) 显著影响牛基因组,水牛比牛拥有更多的ERV. 这些古老的病毒残留物显示了最近的活动,并影响了基因调节.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 病毒学 病毒学
背景情况:
- 内源逆转录病毒 (ERV) 是古老的病毒序列,集成到宿主基因组中.
- 在基因组进化,适应和疾病易感性方面,ERV发挥作用.
- 了解牛类动物的ERV动态对于进化和遗传洞察至关重要.
研究的目的:
- 为了在Bovinae亚家族中对ERV进行全基因组查.
- 调查牛和水牛中的ERV的活动,集成模式和进化起源.
- 分析ERV对蛋白质编码和非编码RNA基因的影响.
主要方法:
- 使用RepeatModeler对来自17个Bovinae物种的49个基因组进行全基因组查.
- 基穆拉分歧分析以评估ERV活性和PCR基因定型用于插入多态性.
- 交叉分析用于绘制基因内ERV整合的地图,以及源代测定源代分析.
主要成果:
- 在Bovinae中确定了22个ERV群体 (20个β-逆转录病毒,2个马逆转录病毒).
- 布巴卢斯布巴利斯 (水牛) 的基因组含有更多的ERV (838个) 比博斯牛 (牛) (575个).
- 五个ERV群体最近表现出活力 (基穆拉分歧<5%),具有特定品种的多态性.
- ERVs富含蛋白质编码和lncRNA基因,以及调控基因位点,但在转录区域中耗尽.
- 埃尔维的起源可追溯到1589万年前,与博维纳的多样化相吻合.
- 在某些物种中,LINE是主要的可转移元素,SINE和DNA转移子的丰度各不相同.
结论:
- 雄牛的基因组比牛的基因组具有显著更高的ERV负载.
- 埃尔维最近表现出活动和品种特定分布,表明对牛的持续影响.
- 通过融入调节区域并影响基因表达,ERVs有助于塑造宿主基因网络.
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