含有铁糖酸盐的藻酸盐水凝促进了Streptococcus hemolyticus中的铁亡,并治愈了链球菌感染
Lishan Yao1, Fukun Xiang1, Shurui Peng1
1School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
International journal of biological macromolecules
|July 12, 2025
概括
铁糖酸盐 (FeGlu) 在"食人细菌"Streptococcus hemolyticus中触发细胞死亡. 一种新型的FeGlu-hydrogel显示出强大的抗菌活性,是对Streptococcus感染的有前途的伤口包裹.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 纳米技术 纳米技术
背景情况:
- 链球菌血溶菌感染是越来越严重的公共卫生问题,因为其发病率和抗菌素耐药性日益增加.
- 迫切需要新的治疗方法来对抗严重的毒性休克综合征和由这种病原体引起的感染.
研究的目的:
- 为了研究 ferrous gluconate (FeGlu) 诱导 S. hemolyticus 细胞死亡的潜力.
- 开发和评估一种含有FeGlu的抗菌水凝,用于治疗链球菌相关的感染.
主要方法:
- 铁制葡萄酸盐 (FeGlu) 被测试了其诱导S. hemolyticus中铁的能力,其特点是铁依赖性,反应性氧物种 (ROS) 爆发和脂质过氧化.
- 通过将FeGlu与藻酸盐 (SA) 进行交叉链接,制备了一种抗菌水凝.
- 评估了水凝的特性,包括流动性,自我愈合,生物相容性,以及对各种类型的菌根杆菌的杀菌功效. 用蛋白质组分析来了解细胞死亡的机制.
主要成果:
- FeGlu诱导了S. hemolyticus中的铁亡,由ROS爆发和脂质过氧化证实,并被铁亡抑制剂抑制.
- FeGlu-水凝表现出优异的流动性,自我愈合和与人类皮肤纤维细胞 (HSF) 细胞的生物相容性.
- 水凝对S. hemolyticus具有异常的杀菌效果,对其他类型的链球菌具有广泛的抗菌作用,包括肺炎链球菌和突变链球菌.
- 蛋白质组分析表明,FeGlu-水凝破坏了跨膜运输,生物巨分子合成和细胞代谢,导致细胞死亡.
结论:
- 铁糖酸盐有效地诱导了Streptococcus hemolyticus中的铁亡.
- 开发的FeGlu-hydrogel是一种安全有效的抗菌生物材料,具有针对Streptococcus物种的广泛活性.
- 这种FeGlu-hydrogel代表了一种有前途的治疗选择,用于管理链球菌相关的感染和伤口护理.
相关概念视频
Antimicrobial Proteins
10.6K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
10.6K
Special Staining Techniques
1.7K
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
1.7K
Microbes in the Production of Fermented Foods
327
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
327
Bacterial Gastroenteritis
88
Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...
88
Inhibitors of Bacterial Protein Synthesis
106
Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
106
Clinical Significance of Antibiotic Resistance
90
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
90


