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在Chlorella pyrenoidosa中L-乳酸脱酶的异构表达:由真核微藻从CO2进行多元 (L-乳酸) 生物合成的开始
Xu Zhang1, Xinxin Meng1, Jiaxin Liang1
1Fermentation Technology Innovation Center of Hebei Province, College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, PR China.
Journal of biotechnology
|July 12, 2025
概括
微藻现在可以使用二氧化碳生产乳酸,这是基于糖的方法的可持续替代品. 这项研究设计了Chlorella pyrenoidosa生物合成乳酸,为环保生物塑料铺平了道路.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微藻工程 微藻工程
背景情况:
- 传统的乳酸生产依赖于基于糖的原料,对粮食安全构成风险.
- 微藻为生物制造中二氧化碳 (CO2) 的利用提供了一个可持续的解决方案.
- 聚乳酸是一种广泛使用的乳酸衍生生物塑料.
研究的目的:
- 建立一个高效的Agrobacterium介导的Chlorella pyrenoidosa (C. pyrenoidosa) 的转化系统.
- 用二氧化碳作为碳源来设计C. pyrenoidosa以生物合成L-乳酸.
- 研究微藻在生产聚L-乳酸前体方面的潜力.
主要方法:
- 在C. pyrenoidosa.中经由农细菌介导的转化.
- 来自Bos taurus,Lactobacillus plantarum和Bacillus coagulans的L-乳酸盐脱酶 (LLDH) 的整合和表达.
- RT-PCR,酶活性测定,同质模型和分子对接以确认基因表达和分析酶功能.
主要成果:
- 成功建立了C. pyrenoidosa的高效转化系统.
- 工程菌株产生的L-乳酸标位为76.64毫克/升 (BCLLDH),153.18毫克/升 (LPLLDH) 和98.21毫克/升 (BTLLDH).
- 验证了转基因稳定性,分子分析支持了酶活性发现.
结论:
- 在C. pyrenoidosa.中成功实现了L-乳酸生物合成.
- 这项研究为真核微藻提供了一条新的途径,用于从CO2中合成L-乳酸.
- 工程微藻提供了一个可持续的平台,用于生产聚L-乳酸的前体.
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