相关实验视频

Updated: Sep 15, 2025

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

人工智能引导的发酵过程调节揭示了Gentamicin C1a过度生产中的代谢重新连接

Feng Xu¹, Lihuan Su², Yuan Wang²

¹State Key Laboratory of Bioreactor Engineering, Qingdao Innovation Institute of East China University of Science and Technology, East China University of Science and Technology, Shanghai 200237, China; National Center of Bio-Engineering & Technology (Shanghai), East China University of Science and Technology, Shanghai 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, Shanghai 200237, China.

Bioresource technology

|July 12, 2025

概括

这项研究引入了一种先进的优化框架,用于甘他C1a的生产,通过集成的人工神经网络-遗传算法 (ANN-GA) 建模和实时发酵控制实现54.6%的标位增加. 该方法提高了生物工艺的效率,并为工业应用提供了可扩展的模型.

科学领域:

生物技术是生物技术.
生物化学工程生物化学工程
代谢工程是代谢工程.

背景情况:

优化工业生物工艺对于有效生产诸如 gentamicin C1a. 这样的有价值化合物至关重要.
传统方法往往缺乏为最大限度地提高产量所需的精度和实时适应性.

研究的目的:

开发和验证一个完整的优化框架,以提高Gentamicin C1a的产量.
利用人工智能和多传感器技术实现实时生物过程控制和代谢途径分析.

主要方法:

开发一个人工神经网络-遗传算法 (ANN-GA) 模型,用于流程优化.
实施多传感器在线监测,使用近红外 (NIR) 和拉曼光谱来实时控制发酵.
应用代谢和代谢流量分析来了解路径的改变.

主要成果:

与传统方法相比,ANN-GA模型显示出更高的预测准确性和适配能力.
实时发酵控制导致 gentamicin C1a 标位增加了 54.6% (385.3 mg/L 与 249.2 mg/L 相比).
代谢分析显示,糖解和TCA循环流量减少,酸路径流量增加,NADPH可用性增强,碳流量向关键前体转向.

结论:

综合框架通过智能优化和实时控制显著提高了甘他素C1a标位.
该研究建立了一个可扩展的范式和优化工业生物工艺的理论基础.
这种方法为提高复杂生物分子的生产效率提供了一个强大的策略.

关键词:

人工神经网络的人工神经网络发酵优化优化发酵的优化遗传算法遗传算法遗传算法代谢流量分析是指代谢流量分析.多传感器监控监控多传感器监控

更多相关视频

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

相关概念视频

Biosynthesis in Bacteria

Biosynthesis in Bacteria

Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...

Microbial Fermentation

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...